Healthcare-associated infections due to multidrug-resistant  Gram-negative bacteria (MDR-GNB) are a leading cause of morbidity and  mortality worldwide. These evidence-based guidelines have been produced  after a systematic review of published studies on infection prevention  and control interventions aimed at reducing the transmission of MDR-GNB.  The recommendations are stratified by type of infection prevention and  control intervention and species of MDR-GNB and are presented in the  form of 'basic' practices, recommended for all acute care facilities,  and 'additional special approaches' to be considered when there is still  clinical and/or epidemiological and/or molecular evidence of ongoing  transmission, despite the application of the basic measures. The level  of evidence for and strength of each recommendation, were defined  according to the GRADE approach.

Keywords: Acinetobacter; Burkholderia; Enterobacteriaceae; Pseudomonas;  Stenotrophomonas; extended-spectrum β-lactamase; guideline; infection  control; multidrug-resistant Gram-negative; outbreak.